Production of Diphtheria Toxin by the Submerged Culture Method

The Production of Diphtheria Toxin

Toxin of sufficient strength to kill a 400-gramme guinea-pig in three days and a half in a dose of 0.cubic centimetre developed in suitable bouillon, contained in ordinary Erlenmeyer flasks, within a period of twenty-four hours. In such boullon the toxin reached its greatest strength in from four to seven days (0.005 cubic centimetre killing a 500-gramme guinea-pig in three days). This period o...

Receptor-mediated endocytosis of diphtheria toxin by cells in culture.

The binding and uptake of fluorescently labeled diphtheria toxin by cells in culture has been examined by using epifluorescence video intensification microscopy. Rhodamine-labeled diphtheria toxin retained significant toxicity on bioassay and in cell culture and was tested for uptake by human WI-38 and mouse 3T3 fibroblasts grown in culture. When added to cells at 37 degrees C, toxin was observ...

Preparation of a low-chloride Medium, for the production of Diphtheria toxin

Improvement of Xylanase Production by Cochliobolus sativus in Submerged Culture

The xylanase production by a new Cochliobolus sativus Cs5 strain was improved under submerged fermentation. The xylanase was induced by xylan and repressed by glucose, sucrose, maltose, xylose, starch and cellulose. Highest enzyme production (98.25 IU/mL) was recorded when wheat straw (4 % by mass per volume) was used as a carbon source after 120 h of incubation. NaNO3 increased xylanase produc...

Production of mold amylases in submerged culture.

During recent years many new and extended uses have been developed for fungal enzymes. Prominent among these are applications in the fields of food manufacturing, textile processing, and in the manufacture of malt beverages and industrial alcohol. Although certain microorganisms are capable of elaborating amylases when grown under submerged conditions either aerobically (Waldmann, 1942) or anae...